0README
enzyme-kinetics-MM
`-- 0README obvious
It is essentially trivial to write an R-language function to fit
the Michaelis-Menten model to enzyme kinetic data.
But why do the work, when then R group has done it for you, and
done it well. The current R distribution has a data set of enzyme
kinetic data (bring into frame with data(Puromycin)) and a built-in
function, "SSmicmen", for the Michaelis-Menten model. For suggestions
on carrying out an MM fit and plotting the results, see ?Puromycin
and ?SSmicmen
Another set of trial data: the enzyme-catalyzed reaction rate, "v",
in reciprocal seconds, as a function of the substrate concentration,
["S"], in mol/l, from kinetic measurements on lysozyme, which is
known to follow the Michaelis-Menten model. The values of the
dependent variable, "v", have equal weights.
S v
.00015 M .105 sec^-1
.00009 .100
.00006 .095
.00006 .083
.00004 .080
.00004 .074
.00003 .061
.00002 .054
.00002 .051
John Rupley
rupley@u.arizona.edu -or- jar@rupley.com
30 Calle Belleza, Tucson AZ 85716 - (520) 325-4533; fax - (520) 325-4991
Dept. Biochemistry & Molecular Biophysics, Univ. Arizona, Tucson AZ 85721